Western blot analysis in rat brain homogenate (15 ug). Overnight incubation PVDF membrane with PSENEN polyclonal antibody (Cat # PAB0141) (15 ug/mL) in PBS containing 1% PBS. Horesradish peroxidase-conjugated anti-rabbit (0.5 ug/mL ; GE-Biosciences) was used for detection.
Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. [provided by RefSeq
hematopoietic stem/progenitor cells protein MDS033,presenilin enhancer 2