Goat antibodies raised against mouse IgG heavy and light chains. Antibodies are adsorbed against human proteins to minimize species cross reactivity, and then affinity isolated using immunogen coupled to agarose beads. The affinity isolated antibodies are then conjugated to horseradish peroxidase (HRP) (Mw = 45.9 KDa).
Purified mouse IgG whole molecule
This product was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Quality Control Testing:
Antibodies are test using Ouchterlony double diffusion to assure reactivity with all mouse isotypes.
The optimal working dilution should be determined by the end user.
In 10 mM PBS, 1.0% BSA, 50% glycerol, pH 7.4
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Evaluation of Abnova Goat Anti-Mouse IgG HRP Conjugate secondary antibody. The western blot transferred membranes loaded with 11 human tissue lysates were incubated with Anti-AKR1A1 (a), or without Anti-AKR1A1 (b) primary antibodies, and followed by the Goat anti-Mouse IgG HRP Conjugate secondary antibody. The signals were detected by chemiluminescent system. The results showed the advantage of no background staining with Abnova secondary antibody in WB application when human tissue samples were used. Abnova WB data were the result of using our Goat Anti-Mouse IgG (H&L) HRP Conjugate secondary antibody. Lane 1, Kidney Lane 2, Liver Lane 3, Colon Lane 4, Stomach Lane 5, Spleen Lane 6, Ovary cancer Lane 7, Lung Cancer Lane 8, Pancreases Lane 9, Placenta Lane 10, Thyroid Lane 11, Tongue Cancer.