MBS (phospho T853) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
MBS (phospho T853) polyclonal antibody (Cat # PAB0009). Western blot analysis of MBS in Hela S3 NE.
Western Blot (Recombinant protein)
Phosphorylation of recombinant MBS on threonine-853 residue by recombinant Rho-kinase 2 in vitro. Western blotting using MBS (phospho T853) polyclonal antibody (Cat # PAB0009).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MBS/MYPT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T853 of human MBS/MYPT1.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Phospho-MBS/MYPT1 Thr853 Antibody detects endogenous MBS/MYPT1 only when phosphorylated at threonine853. The antibody does not recognize other myosin phosphatase regulatory subunit.
Recommend Usage
Western Blot (0.4-1 ug/mL)
ELISA (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
MBS (phospho T853) polyclonal antibody (Cat # PAB0009). Western blot analysis of MBS in Hela S3 NE.Western Blot (Recombinant protein)
Phosphorylation of recombinant MBS on threonine-853 residue by recombinant Rho-kinase 2 in vitro. Western blotting using MBS (phospho T853) polyclonal antibody (Cat # PAB0009).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1R12A
Entrez GeneID
4659GeneBank Accession#
D87930Protein Accession#
BAA22378Gene Name
PPP1R12A
Gene Alias
MBS, MGC133042, MYPT1
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 12A
Omim ID
602021Gene Ontology
HyperlinkGene Summary
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
myosin phosphatase, target subunit 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Chlamydia trachomatis Inclusion Membrane Protein CT228 Recruits Elements of the Myosin Phosphatase Pathway to Regulate Release Mechanisms.
Lutter EI, Barger AC, Nair V, Hackstadt T.
Cell Reports 2013 Jun; 3(6):1921.
Application：IF, Human, HeLa cells.
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Chlamydia trachomatis Inclusion Membrane Protein CT228 Recruits Elements of the Myosin Phosphatase Pathway to Regulate Release Mechanisms.
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