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Last updated: 2016/12/4
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SIRT1 polyclonal antibody

  • Catalog # : PAB0004
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against a recombinant full length SIRT.
  • Immunogen:
  • Recombinant protein corresponding to full length SIRT.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • SIRT1 Antibody detects endogenous levels of SIRT1 protein. The antibody does not cross-react with type I and II HDAC proteins.
  • Recommend Usage:
  • Immunoprecipitation (1-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM phosphate buffer, 150 mM NaCl, pH 7.2 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in human pancreas.
    (SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SIRT1 polyclonal antibody (Cat # PAB0004). Western blot analysis of SIRT1 in Jurkat.
    (SIRT is a phosphoprotein which increases the size of the protein. The actual band size observed may differ from that predicted.)
  • PDF DownloadProtocol Download
  • Immunofluorescence
  • Immunofluorescence
  • Subcellular localization of SIRT1 on 293T cell.
  • Immunoprecipitation
  • Immunoprecipitation
  • Measurement of 293T cell endogenous SIRT1 activity in an immunoprecipitate using SIRT1 polyclonal antibody (Cat # PAB0004) by means of SIRT1/Sir2 Deacetylase Fluorometric (Human) Assay Kit (Cat # KA0105).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • SIRT1
  • Gene Alias:
  • SIR2L1
  • Gene Description:
  • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019691,OTTHUMP00000060745,SIR2alpha,sir2-like 1,sirtuin 1,sirtuin type 1
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