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Last updated: 2016/12/4
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H2AFX (phospho S139) polyclonal antibody

  • Catalog # : PAB0003
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Histone H2A.X.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S139 of human Histone H2A.X.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Phospho-Histone H2A.X Ser139 Antibody detects endogenous Histone H2A.X only when phosphorylated at serine139. The antibody does not recognize other Histone.
  • Recommend Usage:
  • Western Blot (1-2 ug/mL)
    Immunofluorescence (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM phosphate buffer, 150 mM NaCl, pH 7.2 (50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • H2AFX (phospho S139) polyclonal antibody (Cat # PAB0003). Western blot analysis of Histone-H2AX in Jurkat.
  • PDF DownloadProtocol Download
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of MCF-7 that has been treated with 10 uM Camptothecin for 30 min using H2AFX (phospho S139) polyclonal antibody (Cat # PAB0003).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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