Tnf (Rat) Recombinant Protein
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Specification
Product Description
Rat Tnf partial recombinant protein with His tag in C-terminus expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSHMLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL
Host
Escherichia coli
Theoretical MW (kDa)
19.9
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 < 0.2 ng/mL, measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
Storage Buffer
Solution (1 mg/mL) containing 1X PBS, pH 7.4, 10% glycerol, 1 mM DTT.
Storage Instruction
Store at 4°C for 2-4 weeks and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Tnf
Entrez GeneID
24835Protein Accession#
P16599Gene Name
Tnf
Gene Alias
MGC124630, RATTNF, TNF-alpha, Tnfa
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
tumor necrosis factor superfamily member 2|tumor necrosis factor superfamily, member 2|tumor necrosis factor alpha|tumor necrosis factor superfamily, member 2|tumor necrosis factor, alpha (cachetin)|tumor necrosis factor-alpha
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