Tnf (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Tnf recombinant protein expressed in Escherichia coli.
Sequence
MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Host
Escherichia coli
Theoretical MW (kDa)
17.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 < 0.1 ng/mL, measured in the cytolysis of murine L929 cells in the presence of Actinomycin D, corresponding to a specific activity of 1 x 107 IU/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.2. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Tnf
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