TFF3 (Human) Recombinant Protein
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Specification
Product Description
Human TFF3 partial recombinant protein with His tag in N-terminus expressed in Escherichia coli.
Sequence
MKHHHHHHASEEYVGLSANQCAVPAKDRVDCGYPHVTPKECNNRGCCFDSRIPGVPWCFKPLQEAECTF
Host
Escherichia coli
Theoretical MW (kDa)
7.82
Specificity
TFF3 Human, His
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by SDS-PAGE.
Activity
The effective concentration is 10.0-100.0 ng/mL, determined by a chemoattract bioassay using human monocytes.
Storage Buffer
Protein (0.5 mg/mL) was lyophilized from a solution containing 20 mM Tris-HCl, pH 7.5, 150 mM NaCl. Reconstitute the lyophilized powder in ddH2O to 0.5mg/mL, and is not sterile! Please filter the product by an sterile filter before use. In higher concentrations the solubility of this antigen is limited.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TFF3
Entrez GeneID
7033Protein Accession#
Q07654Gene Name
TFF3
Gene Alias
HITF, ITF, TFI, hP1.B
Gene Description
trefoil factor 3 (intestinal)
Omim ID
600633Gene Ontology
HyperlinkGene Summary
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq
Other Designations
intestinal trefoil factor|secretory protein|trefoil factor 3|trefoil factor 3, HITF, human intestinal trefoil factor
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Interactome
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Disease
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