TFF2 (Human) Recombinant Protein
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Specification
Product Description
Human TFF2 partial recombinant protein expressed in Escherichia coli.
Sequence
EKPSPCQCSRLSPHNRTNCGFPGITSDQCFDNGCCFDSSVTGVPWCFHPLPKQESDQCVMEVSDRRNCGYPGISPEECASRKCCFSNFIFEVPWCFFPKSVEDCHY
Host
Escherichia coli
Theoretical MW (kDa)
12
Specificity
TFF2 Human
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 97% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
Determined by the ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/mL.
Storage Buffer
Protein (1 mg/mL) was lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TFF2
Entrez GeneID
7032Protein Accession#
Q03403Gene Name
TFF2
Gene Alias
SML1, SP
Gene Description
trefoil factor 2
Omim ID
182590Gene Ontology
HyperlinkGene Summary
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq
Other Designations
spasmolysin|spasmolytic polypeptide|spasmolytic protein 1
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Interactome
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Disease
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