Inorganic Pyrophosphatase (Yeast)
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Product Description
Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P2O7-4 + H2O + PPase 2HPO4-2). PPase requires divalent metal cation (Mg2+) for the enzymatic activity.
Host
Yeast
Form
Liquid
Activity
Standard reaction: 100 mM Tris-HCl (pH 7.2), 2 mM MgCl2 and 2 mM inorganic pyrophosphate.
Storage Buffer
In 20 mM Tris-HCl, 100 mM KCl, pH 8.0 (0.1 mM EDTA, 1 mM DTT and 50% glycerol).
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Functional Study
In vitro Transcription
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