CSF3 (Human) Recombinant Protein
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Specification
Product Description
Human CSF3 recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
18.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 98% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.1 ng/mL, measured by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), corresponding to a specific activity of 1.0 x 108 IU/mg.
Storage Buffer
Protein was lyophilized after dialysis against 10 mM sodium acetate buffer,pH 4.0. Reconstitute the lyophilized powder in 20 mM AcOH to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — CSF3
Entrez GeneID
1440Protein Accession#
P09919Gene Name
CSF3
Gene Alias
G-CSF, GCSF, MGC45931
Gene Description
colony stimulating factor 3 (granulocyte)
Omim ID
138970Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164327|colony stimulating factor 3|filgrastim|granulocyte colony stimulating factor|lenograstim|pluripoietin
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