Hbegf (Rat) Recombinant Protein
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Specification
Product Description
Rat Hbegf recombinant protein expressed in Escherichia coli.
Sequence
DLEGTDLDLFKVAFSSKPQALATPGKEKNGKKKRKGKGLGKKRDPCLKKYKDYCIHGECRYLKELRIPSCHCLPGYHGQRCHGLTL
Host
Escherichia coli
Theoretical MW (kDa)
9.69999999999999
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 1.0ng/mL, determined by a cell proliferation assay using balb/c 3T3 cells, corresponding to a specific activity of > 1.0 x 106 units/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4, 300 mM NaCl, 5% trehalose. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Hbegf
Entrez GeneID
25433Protein Accession#
Q06175Gene Name
Hbegf
Gene Alias
Dtr, GFHB, Hb-egf, Hegfl
Gene Description
heparin-binding EGF-like growth factor
Gene Ontology
HyperlinkOther Designations
Diphtheria toxin receptor (heparin binding epidermal growth factor - like growth factor)|heparin binding epidermal growth factor-like growth factor|heparin-binding epidermal -growth - like growth factor|heparin-binding epidermal -growth factor
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