Fgf8 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Fgf8 recombinant protein (246 amino acid)expressed in?Escherichia coli.
Sequence
QVRSAAQKRGPGAGNPADTLGQGHEDRPFGQRSRAGKNFTNPAPNYPEEGSKEQRDSVLPKVTQRHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR
Host
Escherichia coli
Theoretical MW (kDa)
28.1
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatography
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 25 ng/mL, determined by the ability to induce proliferation of mouse 3T3 cells in the presence of 0.1 ug/mL heprin, corresponding to a Specific Activity of > 4 x 104Units/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Fgf8
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