Fgf8 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Fgf8 recombinant protein (194 amino acid) expressed in?Escherichia coli.
Sequence
MQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR
Host
Escherichia coli
Theoretical MW (kDa)
22.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 97% as determined by SDS-PAGE.
Activity
ED50 < 20 ng/mL, determined by the ability to induce proliferation of mouse 3T3 cells, corresponding to a Specific Activity of 5 x 104Units/mg.
Storage Buffer
Protein(1 mg/mL) was lyophilized from a solution containing 5mM Na3PO4, pH 7.5, 50 mM NaCl. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Fgf8
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