EPO (Human) Recombinant Protein
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Specification
Product Description
Human EPO partial recombinant protein with His tag in C-terminus expressed in Sf9 cells.
Sequence
APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALRAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDRLEHHHHHH
Host
insect
Theoretical MW (kDa)
19.5
Form
Liquid
Preparation Method
Insect cell (Sf9) expression system
Purification
Chromatography
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 ≤ 0.5 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
Storage Buffer
In? PBS, pH 7.4 (10% glycerol).
Storage Instruction
Stored at 4°C for 2-4 weeks, should be stored at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — EPO
Entrez GeneID
2056Protein Accession#
P01588Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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