EPO (Human) Recombinant Protein
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Specification
Product Description
Human EPO recombinant protein expressed in?HEK293 cells.
Host
Human
Theoretical MW (kDa)
36
Form
Lyophilized
Preparation Method
Mammalian cell (HEK 293) expression system
Purification
Chromatography
Purity
> 95% as determined by SDS-PAGE.
Activity
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is 0.5-2.5ng/mL, corresponding to a specific activity of 400,000-2,000,000 units/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — EPO
Entrez GeneID
2056Protein Accession#
P01588Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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