EPO (Human) Recombinant Protein
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Specification
Product Description
Human EPO recombinant protein expressed in?CHO cells.
Sequence
APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR
Host
Mammals
Theoretical MW (kDa)
21
Form
Lyophilized
Preparation Method
_x005F_x000D__x000D_ Mammalian cell (CHO) expression system_x005F_x000D__x000D_
Purification
Chromatography
Purity
> 98% as determined by (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Activity
The specific activity is 1.5 x 10 5 IU/mg determined by Normocyth-aemic mice.
Storage Buffer
Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — EPO
Entrez GeneID
2056Protein Accession#
P01588Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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