EGF (Human) Recombinant Protein
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Specification
Product Description
Human EGF (P01133) recombinant protein expressed in Escherichia coli.
Sequence
MFPAMPLSSLFANAVLRAQHLHQLAADTYKEFERAYIPEGQRYSIQVNFAHYGNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR.
Host
Escherichia coli
Theoretical MW (kDa)
12.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 x 106 IU/mg.
Storage Buffer
Lyophilized from 10mM HCl.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Protein Accession#
P01133Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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Pathway
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Disease
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