EGF (Human) Recombinant Protein
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Specification
Product Description
Human EGF (P01133) recombinant protein expressed in Escherichia coli.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu.
Host
Escherichia coli
Theoretical MW (kDa)
6.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by HPLC and SDS PAGE
Activity
The ED50, calculated by the dose-dependant proliferation of MDCK cells is < 10ng/mL concentration corresponding to a Specific Activity of 100,000 IU/mg.
Storage Buffer
solution with no additives.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Protein Accession#
P01133Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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