EGF (Human) Recombinant Protein
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Specification
Product Description
Human EGF (Q6QBS2) recombinant protein expressed in Pichia Pastoris.
Sequence
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE.
Host
Yeast
Theoretical MW (kDa)
6
Form
Lyophilized
Preparation Method
Pichia Pastoris expression system
Purity
> 98% by HPLC and SDS PAGE
Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/mL corresponding to a specific activity of 1 x 107 Units/mg.
Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Protein Accession#
Q6QBS2Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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Pathway
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