Il1b (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il1b (P10749, 118 a.a. - 269 a.a.) partial recombinant protein with His tag at N-terminus expressed in Escherichia coli.
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
Host
Escherichia coli
Theoretical MW (kDa)
21
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
The ED50 is < 0.1 ng/mL as determined in a cell proliferation assay using D10.G4.1 mouse helper T cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Tris pH 8.0 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il1b
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