Il1b (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il1b (P10749, 117 a.a. - 269 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
Host
Escherichia coli
Theoretical MW (kDa)
17.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
The ED50 is < 0.9 - 1.3 pg/mL as determined by the dose-dependent proliferation of mouse D10S cells, corresponding to a specific activity of > 1.1 x 106Units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il1b
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