EGF (Human) Recombinant Protein
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Specification
Product Description
Human EGF (P01133, 971 a.a. - 1023 a.a.) partial length recombinant protein expressed in Escherichia coli.
Sequence
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Host
Escherichia coli
Theoretical MW (kDa)
6.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Activity
ED50 is < 0.1 ng/mL, measured by a cell proliferation assay using murine BALB/c 3T3 cells, corresponding to a specific activity of > 1.0 x 107 units/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/ml
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Protein Accession#
P01133Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactome
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