EFNA1 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human EFNA1 (P20827, 19 a.a. - 182 a.a.) partial length recombinant protein with hIgG-His tag expressed in Baculovirus expression system.
Sequence
DRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIHQHEDRCLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHS
Host
Viruses
Theoretical MW (kDa)
46.6
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
ED50 is < 50 ng/mL, measured by the binding ability in a functional ELISA with Human EPHA2.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — EFNA1
Entrez GeneID
1942Protein Accession#
P20827Gene Name
EFNA1
Gene Alias
B61, ECKLG, EFL1, EPLG1, LERK1, TNFAIP4
Gene Description
ephrin-A1
Omim ID
191164Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq
Other Designations
OTTHUMP00000033242|OTTHUMP00000033271|eph-related receptor tyrosine kinase ligand 1|ephrin A1|immediate early response protein B61|ligand of eph-related kinase 1|tumor necrosis factor, alpha-induced protein 4
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Interactome
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Pathway
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