BGN (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human BGN (P21810, 38 a.a. - 368 a.a.) partial length recombinant protein with His tag expressed in Baculovirus expression system.
Sequence
DEEASGADTSGVLDPDSVTPTYSAMCPFGCHCHLRVVQCSDLGLKSVPKEISPDTTLLDLQNNDISELRKDDFKGLQHLYALVLVNNKISKIHEKAFSPLRKLQKLYISKNHLVEIPPNLPSSLVELRIHDNRIRKVPKGVFSGLRNMNCIEMGGNPLENSGFEPGAFDGLKLNYLRISEAKLTGIPKDLPETLNELHLDHNKIQAIELEDLLRYSKLYRLGLGHNQIRMIENGSLSFLPTLRELHLDNNKLARVPSGLPDLKLLQVVYLHSNNITKVGVNDFCPMGFGVKRAYYNGISLFNNPVPYWEVQPATFRCVTDRLAIQFGNYKK
Host
Viruses
Theoretical MW (kDa)
38.3
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
ED50 is < 20 ug/mL, measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — BGN
Entrez GeneID
633Protein Accession#
P21810Gene Name
BGN
Gene Alias
DSPG1, PG-S1, PGI, SLRR1A
Gene Description
biglycan
Omim ID
301870Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival. This gene is a candidate gene for the Happle syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000025928|biglycan proteoglycan|bone/cartilage proteoglycan-I|dermatan sulphate proteoglycan I|small leucine-rich protein 1A
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