DUSP18 (Human) Recombinant Protein
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Specification
Product Description
Human DUSP18 (Q8NEJ0, 1 a.a. - 188 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MTAPSCAFPVQFRQPSVSGLSQITKSLYISNGVAANNKLMLSSNQITMVINVSVEVVNTLYEDIQYMQVPVADSPNSRLCDFFDPIADHIHSVEMKQGRTLLHCAAGVSRSAALCLAYLMKYHAMSLLDAHTWTKSCRPIIRPNSGFWEQLIHYEFQLFGKNTVHMVSSPVGMIPDIYEKEVRLMIPL
Host
Escherichia coli
Theoretical MW (kDa)
23.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 300 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (40% glycerol, 1 mM EDTA, 1 mM DTT, 0.1 mM PMSF)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — DUSP18
Entrez GeneID
150290Protein Accession#
Q8NEJ0Gene Name
DUSP18
Gene Alias
DSP18, DUSP20, LMWDSP20, MGC32658, bK963H5.1
Gene Description
dual specificity phosphatase 18
Omim ID
611446Gene Ontology
HyperlinkGene Summary
DUSP18 is a member of the dual-specificity phosphatase (DSP) family (see DUSP1; MIM 600714), which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues (Hood et al., 2002 [PubMed 12408986]).[supplied by OMIM
Other Designations
low molecular weight dual specificity phosphatase 20
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