PHPT1 (Human) Recombinant Protein
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Specification
Product Description
Human PHPT1 (Q9NRX4, 1 a.a. - 125 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY
Host
Escherichia coli
Theoretical MW (kDa)
15.9
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 120 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate per minute at pH 7.5 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (10% glycerol, 0.2 M NaCl, 2 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — PHPT1
Entrez GeneID
29085Protein Accession#
Q9NRX4Gene Name
PHPT1
Gene Alias
CGI-202, DKFZp564M173, HSPC141, PHP14, bA216L13.10
Gene Description
phosphohistidine phosphatase 1
Omim ID
610167Gene Ontology
HyperlinkGene Summary
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek et al., 2002 [PubMed 12383260]).[supplied by OMIM
Other Designations
1700008C22Rik|OTTHUMP00000022619|phosphohistidine phosphatase 14kDa|sex-regulated protein janus-a
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Pathway
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