DCXR (Human) Recombinant Protein
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Specification
Product Description
Human DCXR (Q7Z4W1, 1 a.a. - 244 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MELFLAGRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC
Host
Escherichia coli
Theoretical MW (kDa)
28
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 1800 pmol/min/ug, and is defined as the amount of enzyme that oxidize 1 pmole of xylitol to L-xylulose per minute at pH 10.0 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (20% glycerol, 50 mM NaCl, 1 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — DCXR
Entrez GeneID
51181Protein Accession#
Q7Z4W1Gene Name
DCXR
Gene Alias
DCR, HCR2, HCRII, KIDCR, P34H, SDR20C1
Gene Description
dicarbonyl/L-xylulose reductase
Omim ID
608347Gene Ontology
HyperlinkGene Summary
DCXR is an enzyme that has both diacetyl reductase (EC 1.1.1.5) and L-xylulose reductase (EC 1.1.1.10) activities (Nakagawa et al., 2002 [PubMed 11882650]).[supplied by OMIM
Other Designations
carbonyl reductase II|human carbonyl reductase 2|kidney dicarbonyl reductase|short chain dehydrogenase/reductase family 20C, member 1
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Pathway
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