Ppif (Rat) Recombinant Protein
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Specification
Product Description
Rat Ppif (P29117, 30 a.a. - 206 a.a.) partial length recombinant protein with His tag expressed in Escherichia coli.
Sequence
CSDGGARGANSSSQNPLVYLDVGADGQPLGRVVLELKADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPAFMCQAGDFTNHNGTGGKSIYGSRFPDENFTLKHVGPGVLSMANAGPNTNGSQFFICTIKTDWLDGKHVVFGHVKEGMDVVKKIESFGSKSGKTSKKIVITDCGQLS
Host
Escherichia coli
Theoretical MW (kDa)
21.2
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
Specific activity is > 1300 nmol/min/ug, and is defined as the amount of enzyme that cleaves 1 nmol of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol, 1 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Ppif
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