NME4 (Human) Recombinant Protein
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Specification
Product Description
Human NME4 (O00746, 33 a.a. - 187 a.a.) partial length recombinant protein with His tag expressed in Escherichia coli.
Sequence
PSWTRERTLVAVKPDGVQRRLVGDVIQRFERRGFTLVGMKMLQAPESVLAEHYQDLRRKPFYPALIRYMSSGPVVAMVWEGYNVVRASRAMIGHTDSAEAAPGTIRGDFSVHISRNVIHASDSVEGAQREIQLWFQSSELVSWADGGQHSSIHPA
Host
Escherichia coli
Theoretical MW (kDa)
19.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
Specific activity is > 120 unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a couple system with PK/LDH.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (40% glycerol, 0.2 M NaCl)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — NME4
Entrez GeneID
4833Protein Accession#
O00746Gene Name
NME4
Gene Alias
NDPK-D, NM23H4, nm23-H4
Gene Description
non-metastatic cells 4, protein expressed in
Omim ID
601818Gene Ontology
HyperlinkGene Summary
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM
Other Designations
NDP kinase D|nucleoside-diphosphate kinase 4
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