FBP2 (Human) Recombinant Protein
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Specification
Product Description
Human FBP2 (O00757, 1 a.a. - 339 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MTDRSPFETDMLTLTRYVMEKGRQAKGTGELTQLLNSMLTAIKAISSAVRKAGLAHLYGIAGSVNVTGDEVKKLDVLSNSLVINMVQSSYSTCVLVSEENKDAIITAKEKRGKYVVCFDPLDGSSNIDCLASIGTIFAIYRKTSEDEPSEKDALQCGRNIVAAGYALYGSATLVALSTGQGVDLFMLDPALGEFVLVEKDVKIKKKGKIYSLNEGYAKYFDAATTEYVQKKKFPEDGSAPYGARYVGSMVADVHRTLVYGGIFLYPANQKSPKGKLRLLYECNPVAYIIEQAGGLATTGTQPVLDVKPEAIHQRVPLILGSPEDVQEYLTCVQKNQAGS
Host
Escherichia coli
Theoretical MW (kDa)
39
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
Specific activity is > 1500 pmol/min/ug, and was obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (10% glycerol, 0.15 M NaCl, 1 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FBP2
Entrez GeneID
8789Protein Accession#
O00757Gene Name
FBP2
Gene Alias
MGC142192
Gene Description
fructose-1,6-bisphosphatase 2
Omim ID
603027Gene Ontology
HyperlinkGene Summary
This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq
Other Designations
D-fructose-1,6-bisphosphate 1-phosphohydrolase|FBPase|OTTHUMP00000021696|fructose-1,6-bisphosphatase isozyme 2|hexosediphosphatase|muscle fructose-bisphosphatase
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Interactome
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Pathway
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