GPT2 (Human) Recombinant Protein
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Specification
Product Description
Human GPT2 (Q8TD30, 1 a.a. - 523 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA
Host
Escherichia coli
Theoretical MW (kDa)
60.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% as analyzed by SDS-PAGE.
Activity
Specific effect is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of GPT2 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Tris-HCl buffer, 0.2M NaCl, pH 7.5 (30% glycerol, 2mM DTT)
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — GPT2
Entrez GeneID
84706Protein Accession#
Q8TD30Gene Name
GPT2
Gene Alias
ALT2
Gene Description
glutamic pyruvate transaminase (alanine aminotransferase) 2
Omim ID
138210Gene Ontology
HyperlinkGene Summary
GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.[supplied by OMIM
Other Designations
alanine aminotransferase 2|glutamic pyruvate transaminase 2|glutamic--alanine transaminase|glutamic--pyruvic transaminase|glutamic-pyruvate transaminase 2
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Interactome
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Pathway
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