CENPM (Human) Recombinant Protein
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Specification
Product Description
Human CENPM (Q9NSP4, 1 a.a. - 180 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL
Host
Escherichia coli
Theoretical MW (kDa)
22.1
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 85% as analyzed by SDS-PAGE.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of CENPM (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Tris-HCl buffer, 0.15M NaCl, pH 8.0 (30% glycerol, 1mM DTT)
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — CENPM
Entrez GeneID
79019Protein Accession#
Q9NSP4Gene Name
CENPM
Gene Alias
C22orf18, CENP-M, MGC861, PANE1, bK250D10.2
Gene Description
centromere protein M
Omim ID
610152Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
OTTHUMP00000028741|proliferation associated nuclear element 1
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