ATP1B2 (Human) Recombinant Protein
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Specification
Product Description
Human ATP1B2 (P14415, 68 a.a. - 290 a.a.) partial recombinant protein with His tag expressed in Baculovirus.
Sequence
ADPDHTPKYQDRLATPGLMIRPKTENLDVIVNVSDTESWDQHVQKLNKFLEPYNDSIQAQKNDVCRPGRYYEQPDNGVLNYPKRACQFNRTQLGNCSGIGDSTHYGYSTGQPCVFIKMNRVINFYAGANQSMNVTCAGKRDEDAENLGNFVMFPANGNIDLMYFPYYGKKFHVNYTQPLVAVKFLNVTPNVEVNVECRINAANIATDDERDKFAGRVAFKLRINKTHHHHHH
Host
Viruses
Theoretical MW (kDa)
26.4
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% as analyzed by SDS-PAGE.
Endotoxin Level
< 1 EU/ug of protein by the LAL method.
Activity
Specific activity is > 3000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of ATP1B2 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol)
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — ATP1B2
Entrez GeneID
482Protein Accession#
P14415Gene Name
ATP1B2
Gene Alias
AMOG
Gene Description
ATPase, Na+/K+ transporting, beta 2 polypeptide
Omim ID
182331Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq
Other Designations
Na+/K+ -ATPase beta 2 subunit|Na, K-ATPase beta-2 polypeptide|adhesion molecule on glia|sodium/potassium-dependent ATPase beta-2 subunit|sodium/potassium-transporting ATPase beta-2 chain
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