IGF1 (Human) Recombinant Protein
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Specification
Product Description
Human IGF1 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA with polyhistidine tag at the C-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 95% as determined by SDS-PAGE.
Endotoxin Level
< 0.01 EU/ ug of protein by the LAL method.
Activity
ED50 is 0.9-3.1 ng/mL, Measured by the induction of MCF-7 cells proliferation. The specific activity of recombinant human IGF-I is > 1.2 x 103 IU/mg.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of FGF11 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IGF1
Entrez GeneID
3479Gene Name
IGF1
Gene Alias
IGFI
Gene Description
insulin-like growth factor 1 (somatomedin C)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene
Other Designations
insulin-like growth factor 1|somatomedin C
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