FGF14 (Human) Recombinant Protein
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Specification
Product Description
Human FGF14 recombinant protein with polyhistidine tag at the N-terminus expressed in Escherichia coli.
Sequence
AAAIASGLIRQKRQAREQHWDRPSASRRRSSPSKNRGLCNGNLVDIFSKVRIFGLKKRRLRRQDPQLKGIVTRLYCRQGYYLQMHPDGALDGTKDDSTNSTLFNLIPVGLRVVAIQGVKTGLYIAMNGEGYLYPSELFTPECKFKESVFENYYVIYSSMLYRQQESGRAWFLGLNKEGQAMKGNRVKKTKPAAHFLPKPLEVAMYREPSLHDVGETVPKPGVTPSKSTSASAIMNGGKPVNKSKT with polyhistidine tag at the N-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 95% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 21 ng/mL, Measured by the induction of 3T3 cells proliferation.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of FGF14 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — FGF14
Entrez GeneID
2259Gene Name
FGF14
Gene Alias
FHF4, MGC119129, SCA27
Gene Description
fibroblast growth factor 14
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A mutation in this gene is associated with autosomal dominant cerebral ataxia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000018661|OTTHUMP00000018662|OTTHUMP00000040726|bA397O8.2|fibroblast growth factor homologous factor 4
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