Products

Product Description

Human HMGB1 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE with polyhistidine tag at the C-terminus.

Host

Escherichia coli

Form

Lyophilized

Preparation Method

Escherichia coli expression system

Purification

Ni-NTA chromatography

Purity

> 98% as determined by SDS-PAGE.

Endotoxin Level

< 0.1 EU/ ug of protein by the LAL method.

Activity

ED₅₀ < 10 ug/mL, Measured by the induction of induce TNF alpha in RAW264.7 cells.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue.

SDS-PAGE analysis of HMGB1 (Human) Recombinant Protein.

Recommend Usage

Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH₂O to 100 ug/mL.

Storage Instruction

Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.

Note

Result of activity analysis