TNFSF11 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
SDS-PAGE analysis of TNFSF11 (Human) Recombinant Protein.
Result of activity analysis
Result of activity analysis
-
Specification
Product Description
Human TNFSF11 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MEKAMVDGSWLDLAKRSKLEAQPFAHLTINATDIPSGSHKVSLSSWYHDRGWAKISNMTFSNGKLIVNQDGFYYLYANICFRHHETSGDLATEYLQLMVYVTKTSIKIPSSHTLMKGGSTKYWSGNSEFHFYSINVGGFFKLRSGEEISIEVSNPSLLDPDQDATYFGAFKVRDID with polyhistidine tag at the C-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 10 ng/mL, Measured by the induction of osteoclast differentiation in RAW264.7 cells.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of TNFSF11 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
-
Applications
Functional Study
SDS-PAGE
-
Gene Info — TNFSF11
Entrez GeneID
8600Gene Name
TNFSF11
Gene Alias
CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf
Gene Description
tumor necrosis factor (ligand) superfamily, member 11
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq
Other Designations
OTTHUMP00000178585|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor ligand superfamily, member 11
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com