MRPL2 (Human) Recombinant Protein
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Specification
Product Description
Human MRPL2 (NP_0570341, 82 a.a. - 202 a.a ) partial recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSGRDHTGRIRVHGIGGGHKQRYRMIDFLRFRPEETKSGPFEEKVIQVRYDPCRSADIALVAGGSRKRWIIATENMQAGDTILNSNHIGRMAVAAREGDAHPLGALPVGTLINNVESEPGR
Host
Escherichia coli
Theoretical MW (kDa)
15.5
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE analysis of MRPL2 (Human) Recombinant Protein
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Phosphate buffer, pH8.0 (1mM EDTA,2mM DTT, 50% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — MRPL2
Entrez GeneID
51069Protein Accession#
Q5T653Gene Name
MRPL2
Gene Alias
CGI-22, MRP-L14, RPML14
Gene Description
mitochondrial ribosomal protein L2
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. [provided by RefSeq
Other Designations
OTTHUMP00000016419
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