CDK2 (Human) Recombinant Protein
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Specification
Product Description
Human CDK2 (NP_001789.2, 1 a.a. - 298 a.a ) full-length recombinant protein with His tag expressed in Baculovirus expression system.
Sequence
MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Host
Viruses
Theoretical MW (kDa)
34.9
Form
Liquid
Preparation Method
Baculovirus expression system
Concentration
0.5mg/mL
Purity
> 90% by SDS-PAGE
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE analysis of CDK2 (Human) Recombinant Protein
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.1mM PMSF, 2 mM DTT, 30% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — CDK2
Entrez GeneID
1017Protein Accession#
P24941Gene Name
CDK2
Gene Alias
p33(CDK2)
Gene Description
cyclin-dependent kinase 2
Omim ID
116953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
Other Designations
cdc2-related protein kinase|cell devision kinase 2|p33 protein kinase
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Interactome
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