GBA3 (Human) Recombinant Protein
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Specification
Product Description
Human GBA3 (NP_066024, 1 a.a. - 469 a.a ) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMAFPAGFGWAAATAAYQVEGGWDADGKGPCVWDTFTHQGGERVFKNQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNKIIDDLLKNGVTPIVTLYHFDLPQTLEDQGGWLSEAIIESFDKYAQFCFSTFGDRVKQWITINEANVLSVMSYDLGMFPPGIPHFGTGGYQAAHNLIKAHARSWHSYDSLFRKKQKGMVSLSLFAVWLEPADPNSVSDQEAAKRAITFHLDLFAKPIFIDGDYPEVVKSQIASMSQKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLIKYQENKKGELGILQDAEIEFFPDPSWKNVDWIYVVPWGVCKLLKYIKDTYNNPVIYITENGFPQSDPAPLDDTQRWEYFRQTFQELFKAIQLDKVNLQVYCAWSLLDNFEWNQGYSSRFGLFHVDFEDPARPRVPYTSAKEYAKIIRNNGLEAHL
Host
Escherichia coli
Theoretical MW (kDa)
56.1
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
0.25mg/mL
Purity
> 85% by SDS-PAGE
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE analysis of GBA3 (Human) Recombinant Protein
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (1 mM DTT, 20% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — GBA3
Entrez GeneID
57733Protein Accession#
Q9H227Gene Name
GBA3
Gene Alias
CBGL1, GLUC, KLrP, MGC104276, MGC126878
Gene Description
glucosidase, beta, acid 3 (cytosolic)
Omim ID
606619Gene Ontology
HyperlinkGene Summary
GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM
Other Designations
cytosolic beta-glucosidase|klotho-related protein
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Interactome
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Pathway
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