SIGLEC15 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human SIGLEC15 (Q6ZMC9, 20 a.a. - 263 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
FVRTKIDTTENLLNTEVHSSPAQRWSMQVPPEVSAEAGDAAVLPCTFTHPHRHYDGPLTAIWRAGEPYAGPQVFRCAAARGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADDRRYFCRVEFAGDVHDRYESRHGVRLHVTAAPRIVNISVLPSPAHAFRALCTAEGEPPPALAWSGPALGNSLAAVRSPREGHGHLVTAELPALTHDGRYTCTAANSLGRSEASVYLFRFHGASGAST
Host
Mammals
Theoretical MW (kDa)
54.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by gel clotting method)
Activity
Immobilized Anti-Siglec15 at 1.0 ug/mL (100 uL/well) can bind Siglec-15, hFc, Human with a EC50 = 5.184 ng/mL and a linear range of 0.064 - 200.0 ng/mL when detected by Mouse Anti - Human IgG Fc-HRP.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SIGLEC15
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