Tnf (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Tnf (P06804, 80 a.a. - 235 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Host
Escherichia coli
Theoretical MW (kDa)
17.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
The ED50 was determined by cytotoxicity assay using L-929 mouse fibrosarcoma cells is < 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 units/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Tnf
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