Cxcl2 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Cxcl2 (P10889, 28 a.a. - 100 a.a.) partial recombinant protein expressed in HEK293 cell.
Sequence
AVVASELRCQCLKTLPRVDFKNIQSLSVTPPGPHCAQTEVIATLKGGQKVCLDPEAPLVQKIIQKILNKGKAN
Host
Mammals
Theoretical MW (kDa)
7.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
The ED50 was determined on Ca2+ mobilization assay in CHO-K1/mCXCR2 (mouse CXCR2 stably expressed in CHO-K1 cells) is < 200.0 ng/mL.
Recommend Usage
Reconstitute the lyophilized powder in ddH?O or PBS up to 100 ug/mL.
Storage Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Storage Instruction
Upon reconstitution, store at 4°C for up to 1 week or store at -20°C up to 3 months.
For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Cxcl2
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