CCL20 (Human) Recombinant Protein
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Specification
Product Description
Human CCL20 (P78556, 27 a.a. - 96 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM
Host
Escherichia coli
Theoretical MW (kDa)
~ 8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
EC50 of human MIP-3 alpha /CCL20 < 200.0 ng/mL, measured by Ca2+ mobilization assay in CHO-K1/G15/hCCR6 cells (human Galpha15 and human CCR6 stably expressed in CHO-K1 cells).
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 53 mM Na2HPO4, 147 mM NaH2PO4, 300 mM NaCl, pH 6.5. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CCL20
Entrez GeneID
6364Protein Accession#
P78556Gene Name
CCL20
Gene Alias
CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38
Gene Description
chemokine (C-C motif) ligand 20
Omim ID
601960Gene Ontology
HyperlinkGene Summary
member 20
Other Designations
exodus-1|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20
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