HGF (Human) Recombinant Protein
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Specification
Product Description
Human HGF (P14210, 23 a.a. - 99 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
PIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWF
Host
Escherichia coli
Theoretical MW (kDa)
~ 8.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
EC50 of human IL-8(77a.a.) < 150.0 ng/mL, measured by Ca2+ mobilization assay in CHO-K1/G15/hCXCR1 cells (human Galpha15 and human CXCR1 stably expressed in CHO-K1 cells).
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
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Applications
Functional Study
SDS-PAGE
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Gene Info — HGF
Entrez GeneID
3082Protein Accession#
P14210Gene Name
HGF
Gene Alias
F-TCF, HGFB, HPTA, SF
Gene Description
hepatocyte growth factor (hepapoietin A; scatter factor)
Omim ID
142409Gene Ontology
HyperlinkGene Summary
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor|hepatopoietin A|lung fibroblast-derived mitogen|scatter factor
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Interactome
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