Fgf2 (Rat) Recombinant Protein
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Specification
Product Description
Rat Fgf2 (P13109, 10 a.a. - 154 a.a.) partial recombinant protein with an N-terminal Gly expressed in Escherichia coli.
Sequence
ALPEDGGGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Host
Escherichia coli
Theoretical MW (kDa)
~ 16.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 0.25 ng/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4.0 × 106 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Fgf2
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