Il6 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il6 (P08505, 25 a.a. - 211 a.a.) partial recombinant protein with His tag expressed in CHO cells.
Sequence
FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT
Host
Mammals
Theoretical MW (kDa)
24 ~ 26
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 3.0 pg/mL, measured in a bioassay using 7TD1 cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il6
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