IGFBP4 (Human) Recombinant Protein
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Specification
Product Description
Human IGFBP4 (P22692, 22 a.a. - 258 a.a.) partial recombinant protein with His tag expressed in HEK 293 Cells.
Sequence
DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE
Host
Human
Theoretical MW (kDa)
30 ~ 35
Form
Lyophilized
Preparation Method
_x005F_x000D__x000D_ Mammalian cell (HEK 293) expression system_x005F_x000D__x000D_
Purity
> 95% as analyzed by SDS-PAGE.
>.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 50.0 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15.0 ng/mL Human IGF-ll.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IGFBP4
Entrez GeneID
3487Protein Accession#
P22692Gene Name
IGFBP4
Gene Alias
BP-4, HT29-IGFBP, IBP4, IGFBP-4
Gene Description
insulin-like growth factor binding protein 4
Omim ID
146733Gene Ontology
HyperlinkGene Summary
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq
Other Designations
IGF-binding protein 4|insulin-like growth factor-binding protein 4
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