TGFA (Human) Recombinant Protein
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Specification
Product Description
Human TGFA (P01135, 40 a.a. - 89 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
VVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA
Host
Human
Theoretical MW (kDa)
8 ~ 10
Form
Lyophilized
Preparation Method
_x005F_x000D__x000D_ Mammalian cell (CHO) expression system_x005F_x000D__x000D_
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 0.4 ng/mL, measured in cell proliferation assay using 3T3 cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — TGFA
Entrez GeneID
7039Protein Accession#
P01135Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactome
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